Liquid Chromatography

Liquid Chromatography (LC), part 1 (Video)

An introduction to liquid chromatography and instrumentation.

Liquid Chromatography (LC), part 2 (Video)

Modes of liquid chromatography - adsorption, partition, ion exchange, and size exclusion.

Exercises Gas Chromatography and Liquid Chromatography

Gas Chromatography and Liquid Chromatography

1)

a) What is the typical physical configuration of a GC column?

b) What types of information pertaining to the column should be reported in a published GC method?

c) How are stationary phase types categorized and name some specific kinds.

2) You are given a sample to analyze by GC. It is a 10-component mixture of environmental pollutants. Outline an approach to developing a GC method for the analysis of this mixture.

3) What are the basic parts of a liquid chromatography (LC) system? Describe the function of each part.

4) a) How are liquid samples typically injected into the flowing stream of an LC system?

b) What is the most popular universal detector used for LC separations? Name and briefly describe one other LC detector.

Exercises LC

Liquid Chromatography

1) Describe the following types of LC separations:
a) Partition chromatography – both normal and reversed phase

b) Adsorption chromatography

c) Ion chromatography

d) Size exclusion/gel permeation chromatography

2) You are given a sample to analyze by LC. It is a 10-component mixture of pharmaceutically active compounds. Outline an approach to developing an LC method for the analysis of this mixture.