Peak width and peak broadening
Abstract Peak broadening plays a critical role in the separation of sample components. When two adjacent peaks are both wide, they will overlap and the separation will be incomplete (poor resolution). Ideal chromatographic peaks are Gaussian and symmetrical. Asymmetrical peaks exhibit either fronting or tailing. Peak fronting or tailing can be caused by poor column quality (degradation of column packing) or by the dead volume of the system. Peak asymmetry can lead to poor separation results. The injection system, the detector, connecting tubings and fittings can all contribute to peak broadening.
KeywordsPeak broadening, peak overlap, peak symmetry, asymmetry factor, fronting, tailing, dead volume.
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