This Topic Circle is for advanced users. It is meant for readers who have achieved a chromatogram and consider, taking into account the demands of the client, whether the separation:
- needs to be improved, then we consider what the requirements for the separation are.
- can be improved, then we will sum up the parameters that affect the separation.
- can be optimized, then we look for systematic approaches (theory and computer programs) and procedures to ensure the best possible separation results possible.
This Topic Circle contains theory as well as a practical approach to optimize separation, including many examples.