Solvent system essentials
Abstract HPLC requires high-purity solvents. Trace dissolved contaminants (especially UV-absorbing molecules) or undissolved particulates in the mobile phase cause significant problems. Filters are used to remove particulates, but these can become blocked. Special consideration must be given to the possibility of bacterial growth or salt crystal formation if buffers are used. Solvent pH is important in many analyses. Solvents must be degassed with helium purging, ultrasound, or vacuum filtration to avoid the development of bubbles in the system.
KeywordsUV absorbance, Solvent reservoir, Purity, Refractive index, Filter, Algae, Salt crystals, Degassing, Vacuum filtration, Ultrasonic treatment, Helium purging, Bacteria, pH, Buffer, Mobile phase
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