UV absorption detectors
Abstract UV and visible absorption detectors are used to detect compounds that contain double bonds that absorb at appropriate wavelengths. Typically, a “monochromatic” band of wavelengths is passed through a cell, and the fraction of light transmitted (T) is measured and converted to an absorption (A = -logT). Variable wavelength UV absorption detectors can scan to take spectra, which provide additional information about components. Diode array detectors measure absorption at all wavelengths in parallel and can provide continuous spectra of the eluting components. UV detection is limited to absorbing samples and to methods whose eluents do not absorb themselves.
KeywordsAbsorption, Ultraviolet, Double bond, Beer’s Law, Transmittance, Absorbance, Extinction coefficient, Bandwidth, Wavelength, Filter, Monochromator, Diode array, Mercury lamp, Deuterium lamp, Tungsten lamp, Grating, Spectrum, Buffer
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