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• The HPLC system, basics
• Introduction to HPLC columns
  • > Historical evolution of dimensions
  • > Column parameters
  • > Column dimensions
  • > Packing a column
  • > Storage of columns
  • > Cartridge systems
  • > Guard columns
  • > Selecting a guard column
• Injection valves
• Solvent system essentials
• Fittings, nut, ferrules
• LC pump types (Click to expand)
• Single stroke piston pump
• Dual piston reciprocating pumps
• Pulse dampener (Animation)
• LC pumps: Reciprocal constant flow (Animation)
• Pneumatic pump (Animation)
• Diaphragm pump
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• How to avoid trouble
• Malfunctions, wear, salt formation
• Before you call the service dept!
• LC Separation modes
• Guidelines for method choice

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