Role of separation techniques

Nico Vonk, Avans+, Breda, The Netherlands

Abstract GC and HPLC both have a place in research, product assessment, and environmental monitoring. While GC works best with analytes below 1000 daltons, HPLC is suited to separating higher molecular weight compounds in order to provide qualitative and quantitative information. HPLC chromatographic separations and separatory systems are characterized by the retention and resolution of analyte peaks, as well as their selectivity and plate number (efficiency).

Keywordsretention, selectivity, resolution, efficiency, plate number, separation, matrix, mobile phase, stationary phase

LevelBasic

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