Nano LC
Remco Swart, Thermo Scientific, NetherlandsAbstract This chapter describes aspects of nanoscale liquid chromatography (nanoLC). NanoLC is a miniaturized HPLC technique performed using columns with an internal diameter (i.d.) between 10-150 µm. The name nanoLC refers to the mobile-phase flow rate which is in the nanoliter per minute range. The main advantage of using smaller i.d. columns in HPLC is the increased detection sensitivity that can be obtained as a result of reduced sample dilution. For that reason nanoLC is ideally suited for the analysis of small sample amounts. In addition, the low flow rate used in nanoLC facilitates easy coupling to mass-spectrometric detectors. The main application of nanoLC is the separation of biomolecules in proteomics and biotechnology.
KeywordsnanoLC, Miniaturization, column switching, proteomics, peptide sequencing
LevelBasic
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