Sample handling: a rational approach
Henk Lingeman, Free University, Free University, NetherlandsAbstract The rationale for sample handling including the choice of the various sample preparation techniques with respect to their sensitivity and selectivity will be discussed.
Looking in a complex matrix for traces in the ng/mL to pg/mL range is just like looking for a needle in haystack. Since the samples are usually complex and ‘dirty’, isolation and quantitation of organic compounds – especially those present at low concentrations- in real life matrix is an analytical challenge. In order to achieve reliable chromatographic / electrophoretic data, relatively ‘pure’ samples must be analyzed, and therefore, ST/SP is an essential part of the separation procedure. The objectives of the method will indicate how much effort should be put into a ST/SP scheme. Some of the factors to consider are the concentration of the analyte, the matrix involved and the specificity required.
LevelBasic
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