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• The Importance of Proteomics
• What is proteomics? (Advanced)
• Peptide mass fingerprinting
• Peptide Fingerprinting and Tandem Mass Spectrometry
  • > The Principal Steps of Mass Fingerprinting
  • > MALDI mechanism
  • > MALDI-TOF-MS
  • > The importance of mass accuracy for protein identification by MALDI-TOF
  • > Protein Identification after 2D PAGE
    Protein Identification after 2D PAGE
  • > Important considerations when identifying proteins
    by mass fingerprinting
  • > Protein identification by MS/MS
  • > Example of a triple quadrupole mass spectrometer
  • > Fragmentation of proteins
  • > Information content of protein analysis by peptide sequencing via MS/MS
• 2D gel electrophoresis (2-DGE)
• Workflow of proteomics experiments (I)
• Tryptic digestion and desalting
• MALDI-TOF & Mascot
• Workflow Fingerprint (II) (Dutch)
• Workflow Fingerprint (II) (E)
• LC – MALDI - MS Coupling
• Nano LC in proteomic analysis

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