Extra-column Band Spreading in UHPLC

Kenneth Fountain, Waters Corporation, Milford, MA, USA

Abstract Extra-column band spreading is the –unwanted- broadening of a chromatographic peak that takes place outside of the active column area in the system, from the point of injection to the point of detection. This type of broadening, which in regular HPLC is of modest importance, becomes very important in the case of UHPLC, since full separation takes place within minutes and peaks have to be very sharp.

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