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Introduction to HPLC columns
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Column packings
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Influence of column parameters
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Selecting and optimizing the column
- > Always a compromise
- > General considerations
- > Analysis time
- > Separation and resolution
- > Effect of retention factor
- > Example of a retention problem
- > Effect of selectivity and efficiency on resolution
- > Example of selectivity
- > An efficiency problem
- > Effect of % modifier
- > Effect of type of modifier on selectivity
- > Optimizing the separation
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Effect of parameters on chromatogram
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Column dimensions and peak broadening
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Separation of ionic compounds
