Influence of column parameters

Abstract In HPLC column dimensions are important in many regards. The interplay between column length, particle diameter and separation quality determines factors such as run time, solvent consumption and peak height. Recently, decreased particle size has flattened van Deemter curves and allowed the development of short, efficient, high-speed columns. The effects of the column parameters e.g. length, particle size on a separation, analysis time and detection limits are described in a compact manner.

KeywordsPlate number (N), Column length, , Wall effect, Radial diffusion, Infinite column, High-speed column, UPLC, Sensitivity, Dilution factor, Injection volume, Retention factor, Peak broadening, Peak volume, van Deemter equation.


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