Transfer of gradient methods

Abstract This chapter deals with the strategy that should be employed to transfer a gradient elution method from a given instrument and given column dimensions to a different instrument and/or different column dimensions. The different parameters affecting the gradient separation include the instrument dwell volume, the column length and the particle diameter. Simple rules are given to avoid change in the quality of the gradient separation while modifying these parameters. The problem of extra column volumes which may lead to additional extra column dispersion is pointed out. An interactive simulated gradient separation is put forward to help for a better understanding of these transfer rules.


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