Mobile phase composition

Abstract The mobile phase together with an RPLC phase forms a separation system. Column type, nature and composition of the mobile phase are decisive factors and key to success or failure of a given separation. Therefore, knowledge of the physico-chemical properties of solvents and mobile phases and the interaction with stationary phases and sample components is indispensable to optimize the mobile phase composition. Also this knowledge is important to understand and predict retention and selectivity processes. This chapters supplies this essential know how.


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